Molecular characterization of class 1, 2 and 3 integrons in clinical multi-drug resistant Klebsiella pneumoniae isolates

Table 1 Primers used for PCR amplification for detection of class 1, 2 and 3 integrons

Gene	Primer	Sequence (5′-3′)	Amplification Product (bp)	Reference
intI1	IntI1-F	TCTCGGGTAACATCAAGG	254	[11]
intI1	IntI-R	AGGAGATCCGAAGACCTC
intI2	IntI2-F	CACGGATATGCGACAAAAAGG	788	[12]
intI2	IntI2-R	TGTAGCAAACGAGTGACGAAATG
intI3	IntI3-F	AGTGGGTGGCGAATGAGTG	600	[13]
intI3	IntI3-R	TGTTCTTGTATCGGCAGGTG
5′CS	5′CS-F	GGCATCCAAGCAGCAAG	Variable	[14]
3′CS	3′CS-R	AAGCAGACTTGACCTGA
attI2	attI2-F	GACGGCATGCACGATTTGTA	Variable	[14]
orfX	orfX-R	GATGCCATCGCAAGTACGAG

ISSN: 2047-2994