Table 1 List of primer pairs and cycling conditions for the nuc, mecA, vanA and vanB genes used in this study
Target gene | nuc | mecA | vanA | vanB |
|---|---|---|---|---|
Primer pairs | 5′-GCGATTGATGGTGATACGGTT-3′ 5′-AGCCAAGCCTTGACGAACTAAAGC-3’ | 5’-AGAAGATGGTATGTGGAAGTTAG--3′ 5′-ATGTATGTGCGATTGTATTGC-3’ | 5’- GGCAAGTCAGGTGAAGATG-3′ 5’ ATCAAGCGGTCAATCAGTTC-3’ | 5’ GTG ACA AAC CGG AGG CGA GGA 3′ 5′ CCG CCA TCC TCC TGC AAA AAA-3’ |
PCR product (bp) | 270 | 583 | 713 | 430 |
Cycling conditions | • Initial denaturation at 94 °C for 5 min. (35 cycles): • Denaturation at 94 °C for 30 s. • Annealing at 55 °C for 30 s. • Polymerization at 72 °C for 1 min. • Final extension step at 72 °C and 10 min. Louie et al., 2002 [9]. | • Initial denaturation at 94 °C for 5 min. (40 cycles): • Denaturation at 94 °C for 30 s. • Annealing at 57 °C for 45 s. • Polymerization at 72 °C for 30 s. • Final extension step at 72 °C and 5 min. Azimian et al., 2012 [12]. | • Initial denaturation at 94 °C for 5 min. (40 cycles): • Denaturation at 94 °C for I min. • Annealing at 55 °C for 1 min. • Polymerization at 72 °C for 2 min. • Final extension step at 72 °C and 5 min. Azimian et al., 2012 [12]. | • Initial denaturation at 94 °C for 10 min. (30 cycles): • Denaturation step at 94 °C and 30 s. • Annealing step at 50 °C and a 45 s. • Polymerization at 72 °C for 30 s. • Final extension step at 72 °C and 10 min. Saadat et al., 2014 [11]. |