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Table 1 List of primer pairs and cycling conditions for the nuc, mecA, vanA and vanB genes used in this study

From: Vancomycin-resistant Staphylococcus aureus isolated from camel meat and slaughterhouse workers in Egypt

Target gene nuc mecA vanA vanB
Primer pairs 5′-GCGATTGATGGTGATACGGTT-3′
5′-AGCCAAGCCTTGACGAACTAAAGC-3’
5’-AGAAGATGGTATGTGGAAGTTAG--3′
5′-ATGTATGTGCGATTGTATTGC-3’
5’- GGCAAGTCAGGTGAAGATG-3′
5’ ATCAAGCGGTCAATCAGTTC-3’
5’ GTG ACA AAC CGG AGG CGA GGA 3′
5′ CCG CCA TCC TCC TGC AAA AAA-3’
PCR product (bp) 270 583 713 430
Cycling conditions • Initial denaturation at 94 °C for 5 min.
(35 cycles):
• Denaturation at 94 °C for 30 s.
• Annealing at 55 °C for 30 s.
• Polymerization at 72 °C for 1 min.
• Final extension step at 72 °C and 10 min.
Louie et al., 2002 [9].
• Initial denaturation at 94 °C for 5 min.
(40 cycles):
• Denaturation at 94 °C for 30 s.
• Annealing at 57 °C for 45 s.
• Polymerization at 72 °C for 30 s.
• Final extension step at 72 °C and 5 min.
Azimian et al., 2012 [12].
• Initial denaturation at 94 °C for 5 min.
(40 cycles):
• Denaturation at 94 °C for I min.
• Annealing at 55 °C for 1 min.
• Polymerization at 72 °C for 2 min.
• Final extension step at 72 °C and 5 min.
Azimian et al., 2012 [12].
• Initial denaturation at 94 °C for 10 min.
(30 cycles):
• Denaturation step at 94 °C and 30 s.
• Annealing step at 50 °C and a 45 s.
• Polymerization at 72 °C for 30 s.
• Final extension step at 72 °C and 10 min.
Saadat et al., 2014 [11].