Table 1 PCR conditions and primer sequences
Primer | Sequence 5′ - 3’ | Amplicon size (bp) | Cycling conditions |
|---|---|---|---|
16SrDNA_F | CCTACGGGNGGCWGCAG | 465 | 1 × 95 °C, 3’ |
16SrDNA_R | GACTACHVGGGTATCTAATCC | 35 × 95 °C, 15″; 55 °C, 20″; 72 °C, 30″ | |
ermB_F | AAGGGCATTTAACGACGAAACTG | 438 | 1 × 95 °C 3’ |
ermB_R | ATTTATCTGGAACATCTGTGGTATG | 40 × 95 °C 15″, 60 °C 20″, 72 °C 30″ | |
ermF_F | CGACACAGCTTTGGTTGAAC | 120 | 1 × 95 °C 3′ |
ermF_R | TTTGACACCACTTTGAAAGGAAA | 40 × 95 °C 15″, 58 °C 20″, 72 °C 30″ | |
mefA | CCTGCAAATGGCGATTATTT | 199 | 1 × 95 °C 3′ |
mefA | AATAGCAAGCACTGCACCAG | 40 × 95 °C 15″, 58 °C 20″, 72 °C 30″ |