Characteristics and diversity of mutations in regulatory genes of resistance-nodulation-cell division efflux pumps in association with drug-resistant clinical isolates of Acinetobacter baumannii

Table 1 Primers used in this study

Target genes	Primer sequence (5′–3′)	Amplicon (bp)	References
PCR and sequencing
OXA-51	F: TAATGCTTTGATCGGCCTTG R: TGGATTGCACTTCATCTTGG	353	[23]
rpoB	F: GTGATAARATGGCBGGTCGT R: CGBGCRTGCATYTTGTCRT	543	[24]
adeB	F: TTAACGATAGCGTTGTAACC R: TGAGCAGACAATGGAATAGT	541	[30]
adeG	F: TTCATCTAGCCAAGCAGAAG R: ATGTGGGCTAGCTAACGGC	652	[9]
adeJ	F: ATTGCACCACCAACCGTAAC R: TAGCTGGATCAAGCCAGATA	463	[30]
adeR	F: ATGAGTGTGTAGGGATAATC R: TACTACAGAAAATAGCGTAAC	961	This study
adeS^a	F: ATGTTAATTAATGTGCGTGGC R: CAGCTTATATGTTAGGTGTCC	1315	This study
adeS	F: ATGAAAAGTAAGTTAGGAATTAGTAAG R: TTAGTTATTCATAGAAATTTTTATG	1074	[15]
baeS1^b	F1: TAAAGATGAAGAAACAATGGAG R1: ATCACCTAACTCATCATTTCG	1024	This study
baeS2^b	F2: CCAGTTTTAGATAAAGAAGATGC R2: ACCAGATGCGCTAACTCGAC	1014	This study
baeR	F: CATTAGGTGGTTTACGTTGTG R: CTGATGTCGCGGTGAGCTG	845	This study
adeL	F: TGGACGGAGCATAAAAGTTTG R: CAATTTCTATACCATAAGTTAAGG	1246	This study
adeN	F: AAACATACCAATGACCATCG R: GTAGCTACTCCATAATAATTG	842	This study
RT-PCR analysis
adeB	F: AACGGACGACCATCTTTGAGTATT R: CAGTTGTTCCATTTCACGCATT	84	[15]
adeG	F: AACTATGCGGTGCTCAAC R: GGTGAATTACTTGGTGATGC	218	[5]
adeJ	F: AGCTGGTGCTATGGGCGTTA R: GCCACCCCATGCAATACG	64	[31]
rpoB	F: GAGTCTAATGGCGGTGGTTC R: ATTGCTTCATCTGCTGGTTG	110	[14]

^aPrimer sets designed in this study for adeS gene, but could not successfully amplify it
^bSince the complete sequence of the baeS gene is 1659 bp, we designed two overlapping primer pairs for full-length amplification and sequencing of this gene

ISSN: 2047-2994