Table 1 Details of the six E. cloacae complex isolates found in the two duodenoscopes and blood cultures of the patient
| Â | Isolate 1 | Isolate 2 | Isolate 3 | Isolate 4 | Isolate 5 | Isolate 6 |
|---|---|---|---|---|---|---|
Date | 14/08/17 | 17/08/17 | 19/10/17 | 19/10/17 | 03/02/18 | 18/07/17 |
Source | Duodenoscope 2 | Duodenoscope 2 | Duodenoscope 2 | Duodenoscope 2 | Patient | Duodenoscope 1 |
Sample site | Channel (brush) | Channel (brush) | Channel (brush) | Forceps elevator | Blood culture | Channel (brush) |
Cluster | ECC cluster 1 | ECC cluster 1 | ECC cluster 1 | ECC cluster 2 | ECC cluster 2 | ECC cluster 1 |
Bacterial quantity | > 100 CFU | 10 CFU | 8 CFU | 90 CFU | 2 positive blood culture bottles | 4 CFU |
WGS species | E. hormaechei subsp. hoffmannii | E. hormaechei subsp. hoffmannii | E. hormaechei subsp. hoffmannii | E. hormaechei subsp. Steigerwaltii | E. hormaechei subsp. Steigerwaltii | E. hormaechei subsp. hoffmannii |
Antibiogram | ||||||
MIC meropenem | ≤ 0.25 | ≤ 0.25 | ≤ 0.25 | ≤ 0.25 | ≤ 0.25 | ≤ 0.25 |
MIC imipenem | ≤ 0.25 | ≤ 0.25 | ≤ 0.25 | ≤ 0.25 | ≤ 0.25 | ≤ 0.25 |
MIC gentamicin | ≤ 1 | ≤ 1 | ≤ 1 | ≤ 1 | ≤ 1 | ≤ 1 |
MIC tobramycin | ≤ 1 | ≤ 1 | ≤ 1 | ≤ 1 | ≤ 1 | ≤ 1 |
MIC cotrimoxazol | ≤ 1 | ≤ 1 | ≤ 1 | ≤ 1 | ≤ 1 | ≤ 1 |
MIC ciprofloxacin | ≤ 0.25 | ≤ 0.25 | ≤ 0.25 | ≤ 0.25 | ≤ 0.5 | ≤ 0.25 |
MIC colistin | ≤ 0.5 | ≤ 0.5 | ≤ 0.5 | ≤ 0.5 | ≤ 0.5 | ≤ 0.5 |
MIC fosfomycin | ≤ 16 | ≤ 16 | ≤ 16 | ≤ 64 | ≤ 64 | ≤ 16 |