Table 1 Primers used for ESBL gene amplification by PCR
Target gene | Primer name | Sequences | Amplicon size (bp) |
|---|---|---|---|
bla SHV | SHV-F | 5’-GCCGGGTTATTCTTATTTGTCCG-3’ | 1007 |
SHV-R | 5’-ATGCCGCCGCCAGTCA − 3’ | ||
bla TEM | TEM-F | 5’-GTATCCGCTCATGAGACAATA-3’ | 966 |
TEM-R | 5’-TCTAAAGTATATATGAGTAAAC-3’ | ||
bla CTX-M | CTX-M-F | 5’-TTTGCGATGTGCAGTACCAGTAA-3’ | 544 |
CTX-M-R | 5’-CGATATCGTTGGTGGTGCCATA-3’ | ||
bla CTX-M-type 1 | CTX-M-1_F | 5’-TCTTCCAGAATAAGGAATCCC-3’ | 909 |
CTX-M-1_R | 5’-CCGTTTCCGCTATTACAAAC-3’ | ||
bla CTX-M-type 2 | CTX-M-2_F | 5’-ATGATGACTCAGAGCATT-3’ | 884 |
CTX-M-2_R | 5’-TTATTGCATCAGAAACCGTG-3’ | ||
bla CTX-M-type 8 | CTX-M-8_F | 5’-TGATGAGACATCGCGTTAAG-3’ | 871 |
CTX-M-8_R | 5’-TAACCGTCGGTGACGATTTT-3’ | ||
bla CTX-M-type 9 | CTX-M-9_F | 5’-ATGGTGACAAAGAGARTGCAA-3’ | 873 |
CTX-M-9_R | 5’-CAGCCCTTCGGCGATGAT-3’ | ||
bla CTX-M-type 14 | CTX-M-14_F | 5’-ATTCAACAAAACCAGTTACAGCCC-3’ | 897 |
CTX-M-14_R | 5’-TTTGAGATGGTGACAAAGAGA-3’ |