- Open Access
Meta-analysis of proportion estimates of Extended-Spectrum-Beta-Lactamase-producing Enterobacteriaceae in East Africa hospitals
Antimicrobial Resistance & Infection Control volume 5, Article number: 18 (2016)
A high proportion of Extended-Spectrum-Beta-Lactamase (ESBL) producing Enterobacteriaceae is causing common infections in all regions of the world. The burden of antibiotic resistance due to ESBL in East Africa is large but information is scarce and thus it is unclear how big the problem really is. To gain insight into the magnitude and molecular epidemiology of ESBL-producing Enterobacteriaceae in East Africa a literature search was performed in PubMed on 31 July 2015 to retrieve articles with relevant information on ESBL.
Methods and results
Meta-analysis was performed to determine overall proportion estimate of ESBL-producing Enterobacteriaceae. A total of 4076 bacterial isolates were included in the analysis. The overall pooled proportion of ESBL-producing Enterobacteriaceae among included surveys done in East African hospitals was found to be 0.42 (95 % CI: 0.34–0.50). Heterogeneity (I2) between countries’ proportions in ESBL was significantly high (96.95 % and p < 0.001). The frequently detected genes encoding ESBL were CTX-M, TEM, SHV and OXA while the most infrequent reported genes were KPC and NDM.
The available studies show a very wide variation in resistance due to ESBL between countries. This highlights a need for active surveillance systems which can help understand the actual epidemiology of ESBL, aid in formulating national or regional guidelines for proper screening of ESBL, and support developing standardized approaches for managing patients colonized with ESBL.
The production of beta-lactamases is the most common mechanism for bacteria to acquire resistance to broad-spectrum beta-lactam antibiotics. These hydrolytic enzymes are encoded by various gene variants. TEM, named after Temoneira, is one of the first enzymes identified in Europe in the 1960s in Escherichia coli . Since then, several other enzymes like CTX, SHV, OXA have been reported in different parts of the world [2–8]. Extended-Spectrum-Beta-Lactamase (ESBL) producing Enterobacteriaceae can be defined as those producing β-lactamases capable of conferring bacterial resistance to the penicillins, first-, second-, and third-generation cephalosporins, and aztreonam (but not the cephamycins or carbapenems) by hydrolysis of these antibiotics, and which are inhibited by β-lactamase inhibitors such as clavulanic acid .
The proportion of ESBL-producing bacteria causing common infections in all regions of the world is high, making antibiotic resistance due to ESBL being a major global public health problem . Patients infected with ESBL not only have an increased risk of treatment failure, sometimes resulting in death, but also require more health-care resources. ESBL bacterial infections are becoming challenging because physicians run out of drug options. Although there might be differences in magnitude depending on region or country, ESBLs used to be considered primarily nosocomial. Currently they can be frequently found in both hospitals and communities, though magnitudes reported in community-based surveys are generally lower [4, 6–8, 11–13]. Several risk factors have been documented to be associated with ESBL acquisition, including: previous hospitalisation, previous use of antibiotics such as third generation cephalosporins, hospital overcrowding, bed sharing when hospitalised, and international travel [3, 14–19].
Due to regional differences in the ESBL proportion and distribution, therapeutic decisions should be based on local guidelines derived from local epidemiological data . In resource-rich countries many surveillance systems have been set up to estimate the burden of bacterial infections including ESBL and to determine risk factors for acquisition of ESBL bacteria as well as the clinical outcomes associated with their infection [10, 21–23]. In East Africa however, only scarce and scattered information is available on ESBL epidemiology and risk factors associated with ESBL bacterial infection. When available, the data mostly originates from hospital-based studies [7, 24–28]. To gain a better insight in the proportion estimates and molecular epidemiology of ESBL-producing Enterobacteriaceae in East Africa, we retrieved available peer-reviewed articles and collated the information in this review article. The PRISMA statement was used to guide the meta-analysis ; the PRISMA checklist is available as supplemental material (Additional file 1).
Materials and methods
Literature search and selection
We defined the East African region as the countries forming the East African Community (Burundi, Kenya, Rwanda, Tanzania and Uganda), with the addition of Ethiopia, based on the UN geographical regions definition. A PubMed search was performed on 31 July 2015 using the search query “(Burundi OR Kenya OR Rwanda OR Tanzania OR Uganda OR Ethiopia) AND (ESBL OR extended-spectrum-beta-lactamase”. A total of 29 potential articles were found. Articles studying non-human subjects, review articles, and articles describing isolates from outside the six countries mentioned above were excluded. A total of 24 articles were included (Fig. 1).
Data extraction and analysis
For data consistency, two people extracted data independently from each article. Whenever there was discordance in the data extracted, consensus was reached by double-checking the article. Data extracted included: name of first authors, year of study (or publication if year of study was not documented), department, target population, isolate source and common species isolated. Other data extracted were the methods used to test for ESBL, the number of Enterobacteriaceae analysed, the number of Enterobacteriaceae positive for ESBL, the gene variants encoding for ESBL, and risk factors for ESBL bacterial infection.
Stata version 13.1 (College Station, Texas 77845 USA) was used to perform meta-analysis of the proportion of ESBL-producing Enterobacteriaceae as described by Nyaga et al. . In the analysis, a random-effects meta-analysis model was used to calculate the pooled (weighted) proportion of ESBL and the I2 statistic (measure of inconsistency). The I2 statistic expresses the percentage of total variation across studies due to heterogeneity. A value of 0 % shows no observed heterogeneity, increasing values indicate increasing heterogeneity.
Distribution of articles describing ESBL in East Africa
The 24 articles reviewed were from cross-sectional hospital-based studies. 4 (16.7 %) were from Ethiopia, 4 (16.7 %) were from Kenya, 12 (50 %) were from Tanzania, 3 (12.5 %) were from Uganda, and 1 (4.2 %) was from Rwanda. No articles were found for Burundi. 18 (75 %) of reviewed articles included patients attending in-patient and out-patient departments, 4 (16.7 %) included patients attending in-patient departments only, and 2 (8.3 %) included patients attending out-patient departments only. 20 (83.3 %) of the reviewed articles studied E. coli plus other species while 19 (79.2 %) studied K. pneumoniae plus other species.
One (4.2 %) article (from Rwanda) investigated the risk factors for infection with ESBL-producing Enterobacteriaceae. In this article, previous use of cephalosporins, ciprofloxacin and hospitalisation were found to be significant risk factors for ESBL bacterial infection.
Laboratory methods used to estimate the proportion of ESBL
Sixteen (66.7 %) of the articles reviewed used Double Disk Synergy Test (DDST) alone; 2 (8.3 %) articles used PCR-sequencing alone while 6 (25 %) articles used both DDST and PCR-sequencing methods to estimate ESBL proportions.
Proportion estimates of ESBL in East Africa
Based on the available data (Fig. 2), East Africa’s overall pooled proportion of ESBL-producing Enterobacteriaceae was 0.42 (95 % CI: 0.34–0.50). Overall heterogeneity was significant (I2 95.95 %, p < 0.001). The pooled proportion of ESBL-producing Enterobacteriaceae in Ethiopia was 0.30 (95 % CI: 0.21–0.38), I2 was 67.98 % and p = 0.02. The pooled proportion of ESBL-producing Enterobacteriaceae in Kenya was 0.47 (95 % CI: 0.23–0.71); I2 was 98.82 % and p < 0.001. The pooled proportion of ESBL-producing Enterobacteriaceae in Tanzania was 0.39 (95 % CI: 0.30–0.48); I2 was 93.16 % and p < 0.001. The pooled proportion of ESBL-producing Enterobacteriaceae in Uganda was 0.62 (95 % CI: 0.38–0.87); I2 was 97.83 % and p < 0.001.
The intra-country heterogeneity is reflected in the range of the estimates of the ESBL proportion. In Tanzania, the median (range) ESBL proportion estimate was 38.8 % (14.2–75.9). In Kenya the median ESBL proportion was 45.8 % (13.1–88.3). Ethiopia recorded median ESBL proportion of 30.9 % (21.9–40.4). In Uganda the recorded median ESBL proportion was 61.7 % (43.8–81.4). The single article for Rwanda reported a 38.3 % ESBL proportion (Fig. 3).
Molecular epidemiology of ESBL in East Africa
Out of 24 reviewed articles, 8 (33.3 %) of which, 4 (50 %) from Tanzania, 3 (37.5 %) from Kenya and 1 (12.5 %) from Uganda had data on ESBL-encoding genes. The predominant ESBL-encoding genes reported were CTX-M, TEM and SHV. Five articles provided proportion estimates for these genes (Table 1). One article reported no quantitative data but identified the same three genes . One article identified CTX-M-15, CMY-2 and AmpC , One article reported the multi resistant and rarer genes VIM (12.3 %), OXA-48 (4.9 %), KPC (3.5 %) and NDM (3.1 %) genes .
The scarcity of studies available from the East African region warrants caution in drawing conclusions. Little information overall is available, with no studies on Burundi and only one on Rwanda. However, this review finds high proportion estimates of ESBL-producing Enterobacteriaceae across hospitals in the East African region.
The overall pooled ESBL proportion estimate for East African hospitals (42 %) is close to estimates for Ghana (49 %), Cameroon (54 %), Gabon (45 %) and Morocco (43 %) [18, 32–34]. This estimate is also close to data reported for China, where a nationwide survey that included 30 hospitals reported over 46 % resistance due to ESBL . However the East African proportion is considerably higher than averages reported for resource-rich countries. For instance, the average ESBL proportion estimates reported in a nationwide hospital survey in Germany for 2012 were in the 10 to 15 % range . In 2012, a study for nine US census regions reported ESBL proportion estimates in the 4 to 12 % range , while a 9-year survey in Japan recorded a proportion estimate of 6.3 % in 2003 increasing to 10–20 % in 2011 . It should be noted that this study focused on community-acquired infections whereas the other studies concerned nosocomial-infections.
In this review, inter-country and inter-study results show a wide and statistically significant degree of variation in proportion estimates (p < 0.05 in all cases). There are several possible factors that may account for the variations seen in this review. The first factor is the difference in sensitivity and specificity between methods used in estimating proportions. Some studies reviewed estimated ESBL proportions using purely phenotypic methods, while others used both phenotypic and molecular-based methods. The study done in Uganda shows the proportion estimate using genotypic methods being higher (44 %) than when the same isolates were screened for ESBL using phenotypic methods (18 %) .
A second factor contributing to the variation in proportion estimates is type of wards or units, site of infection, type of specimen collected and whether patients were attending out-patient or in-patient departments. Hospitalised patients especially in intensive care units are generally at a higher risk of acquiring nosocomial infections, which are likely to be ESBL-producers than patients attending out-patient departments [6, 7, 40–42]. The Rwanda study  reports ESBL proportions of 38 % and 5.9 % among inpatients and outpatients respectively, within a single hospital. Similar findings have been documented in Cameroon where ESBL proportions were 23.1 and 6.7 % among outpatients and healthy volunteers respectively .
Many reports have documented the difference in ESBL proportion estimates between hospitals and congested centers (such as orphanages) versus community-based surveys [15, 16, 43–46]. The lack of any estimates for community-based ESBL carriage in East Africa underscores an urgent need for surveillance in the region. Infection control in hospitals including hand hygiene and rational antibiotic use can be effective measures to stop further spread of the ESBL-producing Enterobacteriaceae in both hospitals and communities.
We noted that few (33.3 %) articles investigated different genes encoding ESBL [6, 12, 24, 31, 39, 47–49]. The most common gene variants in these articles are those encoding CTX, TEM and SHV. However one study in Tanzania reported occurrence of genes such as NDM that confer resistance to Carbapenem . With such little information in hand, it is difficult to devise focused and effective interventions for containing resistance. Data from other parts of the world, notably from resource-rich countries, might not be generalisable to African settings.
Risk factors associated with resistance due to ESBL are key for planning the optimal approach to managing the problem. Of the reviewed articles only the one study done in Rwanda investigated these factors. It was concluded that previous use of ciprofloxacin, third-generation cephalosporin and being an inpatient or hospitalised are risk factors for ESBL carriage . Similarly, several studies done in other sub-Saharan African countries have documented the previous use of antibiotics (ciprofloxacin), previous hospitalisation, overcrowding in hospitals, and bed sharing to be associated with ESBL colonisation [14, 16, 17]. These findings are consistent with several studies done outside of Africa [50–58].
In African settings previous antibiotics use is not entirely dependent on previous hospitalisation. Over-the-counter sale of drugs or self-medication, consumption of counterfeit drugs, improper dosage and non-adherence are very common. These practices unnecessarily fuel the process of positive selection for many types of antibiotic resistance. The majority of sub-Saharan hospitals lack antibiotics resistance monitoring systems, proper antibiotic usage guidelines and proper hospital premises disinfection guidelines. If we do not put strong pragmatic measures in place our hospitals may soon turn into hotspots not only for ESBL-producing Enterobacteriaceae, but also for many other types of resistant microorganisms.
When interpreting the data compiled in this review, a number of limitations must be taken into account. All studies took place in university teaching or referral hospitals, which will have a different case-mix from peripheral health centres, and will generally be located in urban areas. Another source of bias is the fact that no study included community-based findings. As in any meta-analysis, the pooled proportions across studies must be interpreted with care, as protocols for observational studies are not standardised across the studies included in the review. A notable factor contributing to variation is the different mix of in- and outpatients between the studies. Finally, the authors acknowledge that more data on ESBL in East Africa may be available from sources other than those searched for this review.
The burden of antibiotic resistance due to ESBL is present across East African region. Little information overall is available, and close to none for Burundi. The available studies present proportion estimates due to ESBL with a wide degree of variation. The scarcity of data on predictors, clinical outcomes, magnitudes and gene variants encoding resistance due to ESBL-producing Enterobacteriaceae calls for active surveillance systems, which can help understand the current epidemiology of ESBL within the region. Furthermore this can aid in developing national and regional guidelines for proper screening of ESBL as well as developing standardized approaches for managing patients colonized with ESBL-producing Enterobacteriaceae.
Datta N, Kontomichalou P. Penicillinase synthesis controlled by infectious R factors in Enterobacteriaceae. Nature. 1965;208:239–41.
Deng M, Zhu M-H, Li J-J, Bi S, Sheng Z-K, Hu F-S, Zhang J-J, Chen W, Xue X-W, Sheng J-F, Li L-J. Molecular epidemiology and mechanisms of tigecycline resistance in clinical isolates of Acinetobacter baumannii from a Chinese university hospital. Antimicrob Agents Chemother. 2014;58:297–303.
Feglo P, Adu-Sarkodie Y, Ayisi L, Jain R, Spurbeck RR, Springman AC, Engleberg NC, Newton DW, Xi C, Walk ST. Emergence of a novel extended-spectrum-β-lactamase (ESBL)-producing, fluoroquinolone-resistant clone of extraintestinal pathogenic Escherichia coli in Kumasi, Ghana. J Clin Microbiol. 2013;51:728–30.
Xia S, Fan X, Huang Z, Xia L, Xiao M, Chen R, Xu Y, Zhuo C. Dominance of CTX-M-type extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from patients with community-onset and hospital-onset infection in China. PLoS One. 2014;9:e100707.
Tängdén T, Cars O, Melhus A, Löwdin E. Foreign travel is a major risk factor for colonization with Escherichia coli producing CTX-M-type extended-spectrum beta-lactamases: a prospective study with Swedish volunteers. Antimicrob Agents Chemother. 2010;54:3564–8.
Ndugulile F, Jureen R, Harthug S, Urassa W, Langeland N. Extended spectrum beta-lactamases among Gram-negative bacteria of nosocomial origin from an intensive care unit of a tertiary health facility in Tanzania. BMC Infect Dis. 2005;5:86.
Moyo SJ, Aboud S, Kasubi M, Lyamuya EF, Maselle SY. Antimicrobial resistance among producers and non-producers of extended spectrum beta-lactamases in urinary isolates at a tertiary Hospital in Tanzania. BMC Res Notes. 2010;3:348.
Mshana SE, Hain T, Domann E, Lyamuya EF, Chakraborty T, Imirzalioglu C. Predominance of Klebsiella pneumoniae ST14 carrying CTX-M-15 causing neonatal sepsis in Tanzania. BMC Infect Dis. 2013;13:466.
Paterson DL, Bonomo RA. Extended-Spectrum -Lactamases: a clinical update. Clin Microbiol Rev. 2005;18:657–86.
World Health Organization. ANTIMICROBIAL RESISTANCE: global report on surveillance. 2014.
Seni J, Najjuka CF, Kateete DP, Makobore P, Joloba ML, Kajumbula H, Kapesa A, Bwanga F. Antimicrobial resistance in hospitalized surgical patients: a silently emerging public health concern in Uganda. BMC Res Notes. 2013;6:298.
Blomberg B, Jureen R, Manji KP, Tamim BS, Mwakagile DSM, Urassa WK, Fataki M, Msangi V, Tellevik MG, Maselle SY, Langeland N. High Rate of Fatal Cases of Pediatric Septicemia Caused by Gram-Negative Bacteria with Extended-Spectrum Beta-Lactamases in Dar es Salaam, Tanzania. J Clin Microbiol. 2005;43:745–9.
Christopher A, Mshana SE, Kidenya BR, Hokororo A, Morona D. Bacteremia and resistant gram-negative pathogens among under-fives in Tanzania. Ital J Pediatr. 2013;39:1–8.
Lonchel CM, Melin P. Extended-spectrum β -lactamase-producing Enterobacteriaceae in Cameroonian hospitals. Eur J Clin Microbiol Infect Dis. 2013;32:79–87.
Muvunyi CM, Masaisa F, Bayingana C, Mutesa L, Musemakweri A, Muhirwa G, Claeys GW. Decreased susceptibility to commonly used antimicrobial agents in bacterial pathogens isolated from urinary tract infections in Rwanda: need for new antimicrobial guidelines. AmJTrop Med Hyg. 2011;84:923–8.
Lonchel CM, Meex C, Gangoué-Piéboji J, Boreux R, Assoumou M-CO, Melin P, De Mol P. Proportion of extended-spectrum ß-lactamase-producing Enterobacteriaceae in community setting in Ngaoundere, Cameroon. BMC Infect Dis. 2012;12:53.
Isendahl J, Turlej-Rogacka A, Manjuba C, Rodrigues A, Giske CG, Nauclér P. Fecal carriage of ESBL-producing E. coli and K. pneumoniae in children in Guinea-Bissau: a hospital-based cross-sectional study. PLoS One. 2012;7:e51981.
Obeng-Nkrumah N, Twum-Danso K, Krogfelt K a, Newman MJ. High levels of extended-spectrum beta-lactamases in a major teaching hospital in Ghana: the need for regular monitoring and evaluation of antibiotic resistance. AmJTrop Med Hyg. 2013;89:960–4.
Wintersdorff CJH Von, Penders J, Stobberingh EE, Lashof AMLO, Hoebe CJPA, Savelkoul PHM, Wolffs PFG. High Rates of Antimicrobial Drug Resistance Gene Acquisition after International Travel, the Netherlands. 2014;20:649-657
García-Tello A, Gimbernat H, Redondo C, Arana DM, Cacho J, Angulo JC. Extended-spectrum beta-lactamases in urinary tract infections caused by Enterobacteria: Understanding and guidelines for action. Actas urologicas espanolas. 2014;38:678-684.
Muellner P, Pleydell E, Pirie R, Baker MG, Campbell D, Carter PE, French NP. Molecular-based surveillance of campylobacteriosis in New Zealand – from source attribution to genomic epidemiology. Euro Surveill. 2013;18. Available online: http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=20365.
Bathoorn E, Friedrich AW, Zhou K, Arends JP, Borst DM, Grundmann H, Rossen JW. Latent introduction to the Netherlands of multiple antibiotic resistance including NDM-1 after hospitalisation in Egypt, August 2013. Euro Surveill. 2013;18. Available online: http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=20610.
Knetsch C, Lawley T, Hensgens M, Corver J, Wilcox M, Kuijper E. Current application and future perspectives of molecular typing methods to study Clostridium difficile infections. Euro Surveill. 2013;18. Available online: http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=20381.
Mshana SE, Hain T, Domann E, Lyamuya EF, Chakraborty T. Predominance of Klebsiella pneumoniae ST14 carrying CTX-M-15 causing neonatal sepsis in Tanzania. BMC Infect Dis. 2013;13:1.
Blomberg B, Manji KP, Urassa WK, Tamim BS, Mwakagile DSM, Jureen R, Msangi V, Tellevik MG, Holberg-petersen M, Harthug S, Maselle SY, Langeland N. Antimicrobial resistance predicts death in Tanzanian children with bloodstream infections : a prospective cohort study. BMC Infect Dis. 2007;7:1–14.
Mshana SE, Matee M, Rweyemamu M. Antimicrobial resistance in human and animal pathogens in Zambia, Democratic Republic of Congo, Mozambique and Tanzania : an urgent need of a sustainable surveillance system. Ann Clin Microbiol Antimicrob. 2013;12:1.
Meremo A, Mshana SE, Kidenya BR, Kabangila R, Peck R, Kataraihya JB. High prevalence of Non – typhoid salmonella bacteraemia among febrile HIV adult patients admitted at a tertiary Hospital, North-Western Tanzania. Int Arch Med. 2012;5:1.
Blomberg B, Mwakagile DSM, Urassa WK, Maselle SY, Mashurano M, Digranes A, Harthug S, Langeland N. Surveillance of antimicrobial resistance at a tertiary hospital in Tanzania. BMC Public Health. 2004;4:45.
Moher D, Liberati A, Tetzlaff J, Altman DG. Preferred reporting items for systematic reviews and meta-analyses: the PRISMA statement. PLoS Med. 2009;6:e1000097.
Nyaga VN, Arbyn M, Aerts M. Metaprop : a Stata command to perform meta-analysis of binomial data. Arch Public Health. 2014;72:1–10.
Kariuki S, Revathi G, Corkill J, Kiiru J, Mwituria J, Mirza N, Hart CA. Escherichia coli from community-acquired urinary tract infections resistant to fluoroquinolones and extended-spectrum beta-lactams. J Infect Dev Ctries. 2007;1:257–62.
Schaumburg F, Alabi A, Kokou C, Grobusch MP, Köck R, Kaba H, Becker K, Adegnika AA, Kremsner PG, Peters G, Mellmann A. High burden of extended-spectrum β-lactamase-producing Enterobacteriaceae in Gabon. J Antimicrob Chemother. 2013;68:2140–3.
Magoué CL, Melin P, Gangoué-Piéboji J, Okomo Assoumou M-C, Boreux R, De Mol P. Prevalence and spread of extended-spectrum β-lactamase-producing Enterobacteriaceae in Ngaoundere, Cameroon. Clin Microbiol Infect. 2013;19:E416–20.
Girlich D, Bouihat N, Poirel L, Benouda A, Nordmann P. High rate of faecal carriage of extended-spectrum β-lactamase and OXA-48 carbapenemase-producing Enterobacteriaceae at a university hospital in Morocco. Clin Microbiol Infect. 2014;20:350–4.
Zhang J, Zheng B, Zhao L, Wei Z, Ji J, Li L, Xiao Y. Nationwide high prevalence of CTX-M and an increase of CTX-M-55 in Escherichia coli isolated from patients with community-onset infections in Chinese county hospitals. BMC Infect Dis. 2014;14:659.
Leistner R, Schröder C, Geffers C, Breier A-C, Gastmeier P, Behnke M. Regional distribution of nosocomial infections due to ESBL-positive Enterobacteriaceae in Germany: data from the German National Reference Center for the Surveillance of Nosocomial Infections (KISS). Clin Microbiol Infect. 2015;21(255):e1–5.
Castanheira M, Farrell SE, Krause KM, Jones RN, Sader HS. Contemporary diversity of β-lactamases among Enterobacteriaceae in the nine U.S. census regions and ceftazidime-avibactam activity tested against isolates producing the most prevalent β-lactamase groups. Antimicrob Agents Chemother. 2014;58:833–8.
Chong Y, Shimoda S, Yakushiji H, Ito Y, Miyamoto T, Kamimura T, Shimono N, Akashi K. Community spread of extended-spectrum β-lactamase-producing Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis: a long-term study in Japan. J Med Microbiol. 2013;62(Pt 7):1038–43.
Moses A, Bwanga F, Boum Y, Bazira J. Prevalence and Genotypic Characterization of Extended-Spectrum Beta-Lactamases Produced by Gram Negative Bacilli at a Tertiary Care Hospital in Rural South Western Uganda. Br Microbiology Res J. 2014;4:1541–50.
Manyahi J, Matee MI, Majigo M, Moyo S, Mshana SE, Lyamuya EF. Predominance of multi-drug resistant bacterial pathogens causing surgical site infections in Muhimbili National Hospital, Tanzania. BMC Res Notes. 2014;7:500.
Mshana SE, Imirzalioglu C, Hain T, Domann E, Lyamuya EF, Chakraborty T. Multiple ST clonal complexes, with a predominance of ST131, of Escherichia coli harbouring blaCTX-M-15 in a tertiary hospital in Tanzania. Clin Microbiol Infect. 2011;17:1279–82.
Nelson E, Kayega J, Seni J, Mushi MF, Kidenya BR, Hokororo A, Zuechner A, Kihunrwa A, Mshana SE. Evaluation of existence and transmission of extended spectrum beta lactamase producing bacteria from post-delivery women to neonates at Bugando Medical Center, Mwanza-Tanzania. BMC Res Notes. 2014;7:279.
Suh Yah C. Plasmid-encoded multidrug resistance: a case study of Salmonella and Shigella from enteric diarrhea sources among humans. Biol Res. 2010;43:141–8.
Sire J-M, Nabeth P, Perrier-Gros-Claude J-D, Bahsoun I, Siby T, Macondo EA, Gaye-Diallo A, Guyomard S, Seck A, Breurec S, Garin B. Antimicrobial resistance in outpatient Escherichia coli urinary isolates in Dakar, Senegal. J Infect Dev Ctries. 2007;1:263–8.
Bourjilat F, Bouchrif B, Dersi N, Claude JDPG, Amarouch H, Timinouni M. Emergence of extended-spectrum beta-lactamases-producing Escherichia coli in community-acquired urinary infections in Casablanca, Morocco. J Infect Dev Ctries. 2011;5:850–5.
Frank T, Arlet G, Gautier V, Talarmin A, Bercion R. Extended-spectrum beta-lactamase-producing Enterobacteriaceae, Central African Republic. Emerg Infect Dis. 2006;12:863–5.
Mushi MF, Mshana SE, Imirzalioglu C, Bwanga F. Carbapenemase Genes among Multidrug Resistant Gram Negative Clinical Isolates from a Tertiary Hospital in Mwanza, Tanzania. BioMed Res Int. 2014;2014:303104.
Maina D, Revathi G, Kariuki S, Ozwara H. Genotypes and cephalosporin susceptibility in extended-spectrum beta-lactamase producing enterobacteriaceae in the community. J Infect Dev Ctries. 2012;6:470–7.
Kiiru J, Kariuki S, Goddeeris BM, Butaye P. Analysis of β-lactamase phenotypes and carriage of selected β-lactamase genes among Escherichia coli strains obtained from Kenyan patients during an 18-year period. BMC Microbiol. 2012;12:155.
Gudiol C, Calatayud L, Garcia-Vidal C, Lora-Tamayo J, Cisnal M, Duarte R, Arnan M, Marin M, Carratalà J, Gudiol F. Bacteraemia due to extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-EC) in cancer patients: clinical features, risk factors, molecular epidemiology and outcome. J Antimicrob Chemother. 2010;65:333–41.
Søraas A, Sundsfjord A, Sandven I, Brunborg C, Jenum P a. Risk factors for community-acquired urinary tract infections caused by ESBL-producing enterobacteriaceae--a case–control study in a low prevalence country. PLoS One. 2013;8:e69581.
Superti SV, Augusti G, Zavascki AP. Risk factors for and mortality of extended-spectrum-β-lactamase-producing Klebsiella pneumoniae and Escherichia coli nosocomial bloodstream infections. Rev Inst Med Trop Sao Paulo. 2009;51:211–6.
Cornejo-Juárez P, Pérez-Jiménez C, Silva-Sánchez J, Velázquez-Acosta C, González-Lara F, Reyna-Flores F, Sánchez-Pérez A, Volkow-Fernández P. Molecular analysis and risk factors for Escherichia coli producing extended-spectrum β-lactamase bloodstream infection in hematological malignancies. PLoS One. 2012;7:e35780.
Kaya O, Akcam FZ, Gonen I, Unal O, Ceylan T. Original Article Risk factors for bacteremia due to extended-spectrum beta-lactamase- producing Escherichia coli in a Turkish hospital. J Infect Dev Ctries. 2013;2011(7):507–12.
Han JH, Kasahara K, Edelstein PH, Bilker WB, Lautenbach E, Presbyterian P. Risk Factors for Infection or Colonization with CTX-M Extended-. Antimicrob Agents Chemother. 2012;56:5575–80.
Demirdag K, Hosoglu S. Epidemiology and risk factors for ESBL-producing Klebsiella pneumoniae : a case control study. J Infect Dev Ctries. 2010;2010(4):717–22.
Silva N, Oliveira M, Bandeira AC, Brites C. Risk factors for infection by extended-spectrum beta-lactamase producing Klebsiella pneumoniae in a tertiary hospital in Salvador, Brazil. Braz J Infect Dis. 2006;10:191–3.
Tumbarello M, Spanu T, Sanguinetti M, Citton R, Montuori E, Leone F, Fadda G, Cauda R. Bloodstream Infections Caused by Risk Factors, Molecular Epidemiology, and Clinical Outcome. Antimicrob Agents Chemother. 2006;50:498–504.
Kayange N, Kamugisha E, Mwizamholya DL, Jeremiah S, Mshana SE: Predictors of positive blood culture and deaths among neonates with suspected neonatal sepsis in a tertiary hospital, Mwanza-Tanzania. BMC pediatrics 2010;10:1–9.
Kateregga JN, Kantume R, Atuhaire C, Lubowa MN, Ndukui JG: Phenotypic expression and prevalence of ESBL-producing Enterobacteriaceae in samples collected from patients in various wards of Mulago Hospital, Uganda. BMC pharmacology & toxicology 2015;16:1–6.
We are thankful to GK and FM for their advice and initial inputs to this manuscript.
The authors declare that they have no competing interests.
TS and GK conceived the idea. TS and MZ gathered data, analyzed them and prepared manuscript draft. All authors read, revised and approved the final manuscript.
Rights and permissions
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
About this article
Cite this article
Sonda, T., Kumburu, H., van Zwetselaar, M. et al. Meta-analysis of proportion estimates of Extended-Spectrum-Beta-Lactamase-producing Enterobacteriaceae in East Africa hospitals. Antimicrob Resist Infect Control 5, 18 (2016). https://doi.org/10.1186/s13756-016-0117-4
- Antibiotic resistance
- East Africa