Fig. 2

Minimum spanning tree of 34 vancomycin-resistant E. faecium isolates from 33 children and one mother. The number of varying alleles is shown next to the black lines. Colouring was based on cgMLST (analysis of 1423 genes of the nuclear genome). Grey areas connect isolates that belong to a cluster based on the definitions of SeqSphere + (maximum allele difference = 15 alleles). Dashed lines include isolates that belong to one sequence type (ST). ST = sequence type (multilocus sequence typing (MLST), analysis of 7 housekeeping genes), CT = complex type (core genome MLST (cgMLST)). SeqSphere option: pairwise ignoring missing values. Task templates: E. faecium cgMLST v1.1; E. faecium MLST v1.0. *adk: Novel allele, ST may indicate nearest ST. MLST-Finder: http://www.genomicepidemiolog