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Risk for subsequent infection and mortality after hospitalization among patients with multidrug-resistant gram-negative bacteria colonization or infection



Risks for subsequent multidrug-resistant gram-negative bacteria (MDRGNB) infection and long-term outcome after hospitalization among patients with MDRGNB colonization remain unknown.


This observational study enrolled 817 patients who were hospitalized in the study hospital in 2009. We defined MDRGNB as a GNB resistant to at least three different antimicrobial classes. Patients were classified into MDRGNB culture-positive (MDRGNB-CP; 125 patients) and culture-negative (MDRGNB-CN; 692 patients) groups based on the presence or absence of any MDRGNB identified from either active surveillance or clinical cultures during index hospitalization. Subsequent MDRGNB infection and mortality within 12 months after index hospitalization were recorded. We determined the frequency and risk factors for subsequent MDRGNB infection and mortality associated with previous MDRGNB culture status.


In total, 129 patients had at least one subsequent MDRGNB infection (MDRGNB-CP, 48.0%; MDRGNB-CN, 10.0%), and 148 patients died (MDRGNB-CP, 31.2%; MDRGNB-CN, 15.9%) during the follow-up period. MDR Escherichia coli and Acinetobacter baumannii were the predominant colonization microorganisms; patients with Proteus mirabilis and Pseudomonas aeruginosa had the highest hazard risk for developing subsequent infection. After controlling for other confounders, MDRGNB-CP during hospitalization independently predicted subsequent MDRGNB infection (hazard ratio [HR], 5.35; 95% confidence interval [CI], 3.72–7.71), all-cause mortality (HR, 2.42; 95% CI, 1.67–3.50), and subsequent MDRGNB infection-associated mortality (HR, 4.88; 95% CI, 2.79–8.52) after hospitalization.


Harboring MDRGNB significantly increases patients’ risk for subsequent MDRGNB infection and mortality after hospitalization, justifying the urgent need for developing effective strategies to prevent and eradicate MDRGNB colonization.

Key points

Harboring multidrug-resistant gram-negative bacteria (MDRGNB) significantly increases patients’ risk for subsequent infection after hospital discharge. The association between MDRGNB colonization and increased long-term mortality further justifies the need for effective, collaborative strategies to prevent and eradicate MDRGNB colonization.


The emergence and spread of multidrug-resistant gram-negative bacteria (MDRGNB) have become a major public health threat globally [1, 2]. Infections with MDRGNB are associated with higher hospital cost, prolonged hospitalization, and mortality [3,4,5,6,7]. Acquisition and infection of MDRGNB are common among hospitalized patients, especially for critically ill patients who were vulnerable to high MDRGNB selection pressure following extensive antimicrobial therapy [8,9,10,11,12]. The risk for subsequent infection was significantly higher for hospitalized patients with initial antimicrobial-resistant GNB colonization than patients without colonization [8, 13,14,15]. Approximately 9.1–39% of inpatients who were initially colonized with various antimicrobial-resistant GNB developed subsequent infection during the same hospital stay [8, 10, 12,13,14]. Therefore, the effect of initial MDRGNB colonization on the risk for subsequent infection and clinical outcomes among hospitalized patients is well documented, and it significantly influences the recommendations for controlling and treating MDRGNB infections in hospital settings.

In contrast to in-hospital settings, decreased risk for MDRGNB colonization and infection over time following hospital discharge was usually deemed as a matter of course. Although the clearance of MDRGNB colonization of a patient after being away from nosocomial, high-antibiotic pressure environment has been demonstrated [16], prolonged effect from persistent MDRGNB colonization remains a potential contributing factor for subsequent MDRGNB infections and threats on the treatment outcomes of community patients with recent hospitalization history. However, the effects of MDRGNB colonization on subsequent infection and long-term outcome of patients after hospitalization have not been comprehensively explored.

In this study, we used any positive culture for MDRGNB, either from active surveillance cultures on index hospitalization admission or decision-driven clinical cultures during index hospitalization, to identify patients with potential MDRGNB colonization, irrespective of the antibiotic treatment history. Then, we hypothesized that MDRGNB colonization along with certain patient characteristics synergistically affects the risk for subsequent MDRGNB associated infection and long-term mortality after hospital discharge. We also hypothesized that there exists different species-specific and isolation site-specific colonization effects that contribute to the different risk for subsequent MDRGNB infection. Therefore, this study aimed to provide data on the characteristics of subsequent infection pattern and outcome effects associated with prior MDRGNB colonization or infection to help first-line physicians in making treatment decisions for patients with community-onset infection.


Study design, setting, and patients

The National Taiwan University Hospital is a 2200-bed teaching hospital that provides both primary and tertiary care in northern Taiwan. This retrospective study used an adult patient cohort of one prospective study that recruited 995 patients in the emergency department who received active microbiological surveillance cultures for the development a MDRGNB prediction model on patients’ hospital admission (index hospitalization) [17]. The results of active surveillance cultures, which included anterior nares swab, posterior pharyngeal wall (throat) swab, urine, and areas of skin breakdown if presented, and all clinical cultures during index hospitalization of the 995 patients were recorded to determine the MDRGNB colonization status on admission and before hospital discharge. Among the 995 patients, 118 (11.6%) died during hospitalization and 60 (6.0%) did not have any outpatient department (OPD) follow-up record after hospital discharge; hence, they were excluded from this study. Therefore, only 817 patients who survived the index hospitalization discharge and had at least one OPD follow-up record were finally enrolled. We then conducted this observational study to investigate the risk for subsequent MDRGNB infections and mortality in these patients during 12 months after discharge from the index hospitalization (Appendix).

Definition of MDRGNB and determination of colonization status

We defined MDRGNB as the presence of Enterobacteriaceae or glucose non-fermentative gram-negative bacilli (NFGNB) that are resistant to at least three different antimicrobial classes. For Enterobacteriaceae, MDR was defined as resistance to at least three classes of the following agents: third- or fourth-generation cephalosporins, aminoglycosides, fluoroquinolones, and ampicillin/sulbactam. For NFGNB, MDR was defined as resistance to at least three classes of the following agents: antipseudomonal cephalosporins (ceftazidime or cefepime), aminoglycosides, fluoroquinolones (levofloxacin or ciprofloxacin), antipseudomonal penicillins (ticarcillin-clavulanic acid or piperacillin-tazobactam), and carbapenem (imipenem or meropenem) [1, 18]. Patients with and without any positive culture for MDRGNB from either active surveillance cultures on admission or decision-driven clinical cultures during index hospitalization were classified as MDRGNB culture-positive (MDRGNB-CP) and MDRGNB culture-negative (MDRGNB-CN) groups, respectively.

Data collection and information on variables

For all study patients, clinical data, including age, sex, preexisting comorbidities, antibiotic exposure, intensive care unit (ICU) admission, receiving of tracheal intubation, length of hospital stay (LOS), clinical cultures within 1 year prior to index hospitalization, active surveillance cultures on admission, and decision-driven clinical cultures obtained during index hospitalization were prospectively obtained from medical records [17]. Follow-up data 12 months after index hospitalization were retrospectively collected from the hospital and OPD electronic medical records, including repeated hospitalization, subsequent clinical culture results, occurrence of culture-confirmed MDRGNB infection, and mortality.

Several comorbid medical conditions were investigated. Malignancy included either an active malignant solid tumor or hematological disease. Severity of preexisting comorbidities was assessed using a modified Charlson comorbidity score [19, 20]. The diagnosis of MDRGNB infection of a study patient was independently evaluated by two investigators based on clinical, radiographic, and microbiological findings and National Nosocomial Infections Surveillance criteria [21, 22]. A third investigator confirmed and finalized the decision if the two investigators did not agree on the diagnosis of MDRGNB infection. All subsequent MDRGNB infections were described according to the number of days between the onset of infection and the index hospitalization discharge date, source of infection, and causative microorganism. For patients with multiple episodes of subsequent MDRGNB infection during the 12 months follow-up period, only the first episode was analyzed, because the causative microorganism of later episodes of MDRGNB infection could be a new colonization acquired during subsequent hospitalization for the first episode of MDRGNB infection treatment. MDRGNB-associated mortality was defined as MDRGNB bacteremia occurring within 7 days of death or active MDRGNB infection at the time of death [23].

Statistical analyses

Means (±SD) were calculated for continuous variables, and percentages were used for categorical variables. Independent Student’s t-test or Mann–Whitney U test was used to compare continuous variables, and Chi-square or Fisher’s exact test was used to analyze categorical variables. Kaplan–Meier method and log-rank tests were used to compare the cumulative probability of the first episode of subsequent MDRGNB infection and survival after index hospitalization of both the study groups. We screened for variables with P values of ≤0.2 using univariate analysis and included these as candidate variables in the multivariate Cox regression model. We then used stepwise selection of these variables to investigate independent risk factors associated with subsequent MDRGNB infection and 1-year survival. The risks for subsequent infection due to the same MDRGNB species or isolation site were presented as hazard risk between patients with positive culture for the indicated MDRGNB species or isolation site and those without any MDRGNB culture during index hospitalization. Data were analyzed using SAS 9.4 (SAS Institute, Cary NC). All P values are two sided, and findings with P values of < 0.05 were considered statistically significant.


In total, 817 patients were enrolled in this study, including 125 patients with at least one positive MDRGNB from surveillance (71 patients), clinical (93 patients), or both (39 patients) cultures. The microbiological distribution of MDRGNB species is detailed in Table 1. Escherichia coli was the most predominant MDRGNB isolate (37.6% [47/125]), followed by Acinetobacter baumannii (25.6% [32/125]), Pseudomonas aeruginosa (17.6% [22/125]), and Klebsiella pneumoniae (16.8% [21/125]) during the index hospitalization. Demographic and clinical characteristics of the 817 study patients with and without positive MDRGNB culture during index hospitalization are summarized in Table 2. MDRGNB-CP patients were older, had longer index hospitalization LOS, and had higher percentage of prior healthcare-associated exposure or MDRGNB culture before index hospitalization. MDRGNB-CP patients also had a higher percentage of having cardiovascular diseases and a bed-ridden status, presence of a pressure sore or indwelling urinary catheter, receiving ICU care and tracheal intubation, and antibiotic exposure during index hospitalization.

Table 1 Bacteriology and Culture Site of Multidrug-resistant Gram-negative Bacteria (MDRGNB) Isolates Identified during Index Hospitalization and Within 1 year after Index Hospitalization Discharge
Table 2 Clinical Characteristics of Study Patients by the Detection of Multidrug-resistant Gram-negative Bacteria (MDRGNB) in Either Surveillance of Clinical Culture during Index Hospitalization

After discharge from index hospitalization, 129 patients (60 MDRGNB-CP and 69 MDRGNB-CN) had at least one episode of subsequent MDRGNB infection during the 12-month follow-up period. The median duration from index hospitalization discharge to the first subsequent MDRGNB infection episode was 74 (range, 2–354) days for all the 129 patients with subsequent infection episodes, 44.5 (range, 2–354) days for the 60 MDRGNB-CP patients, and 108 (range, 10–352) days for the 69 MDRGNB-CN patients. E. coli remained the most predominant causative microorganism of subsequent MDR-GNB infections in both MDRGNB-CP and MDRGNB-CN groups (Table 1). Among the 129 patients with subsequent MDRGNB infections, 5 had two concomitant MDRGNB infection foci (respiratory and urinary tracts [4], respiratory tract and intra-abdominal [1]). The lower respiratory tract was the most prevalent primary focus of subsequent MDRGNB infection (66.7% [86/129]), followed by urinary tract (24.8% [32/129]), intra-abdominal area (7.8% [10/129]), other infection foci (2.3% [3/129]), and primary bacteremia (2.3% [3/129]). Among the 60 subsequent MDRGNB infection episodes from the 125 MDRGNB-CP patients, 41 (68.3%) were caused by the same MDR bacterial species, 43 (71.7%) had the infection site concordant to prior MDRGNB culture site, and 33 (55.0%) had the causative MDR bacterial species and culture site concordant to prior MDRGNB culture during index hospitalization. MDRGNB-CP patients had significantly higher cumulative probability of developing subsequent MDRGNB infection than MDRGNB-CN patients (log-rank test, P < 0.001) (Fig. 1a). Multivariate Cox regression analysis showed the positive MDRGNB culture during index hospitalization was an independent predictor for subsequent MDRGNB infection (hazard ratio [HR], 5.35; 95% confidence interval [CI], 3.72–7.71), after controlling other potential confounders, such as age (HR, 1.02; 95% CI, 1.01–1.03), malignancy (HR, 1.66; 95% CI, 1.12–2.47), congestive heart failure (HR, 1.70; 95% CI, 1.08–2.68), chronic obstructive pulmonary disease (HR, 1.60; 95% CI, 1.03–2.48), and antibiotics exposure during index hospitalization (HR, 2.20; 95% CI, 1.25–3.90) (Table 3).

Fig. 1

Kaplan–Meier curves at 1 year for a subsequent MDRGNB infection, b all-cause mortality, and c MDRGNB infection-associated mortality after discharge, stratified by index hospitalization MDRGNB culture result. Abbreviations: MDRGNB, multidrug-resistant gram-negative bacteria; MDRGNB-CN, multidrug-resistant gram-negative bacteria culture negative; MDRGNB-CP, multidrug-resistant gram-negative bacteria culture positive

Table 3 Univariate and Multivariate Cox Regression Analysis for the Risk for Subsequent Multidrug-resistant Gram-negative Bacteria (MDRGNB) Infection after Hospital Discharge

Analysis for species-specific risk for subsequent infection after index hospitalization discharge showed that the hazard risk in patients with initial MDR Proteus mirabilis culture was higher (HR, 231.29; 95% CI, 32.10–1666.31) than those without any MDRGNB culture, followed by MDR P. aeruginosa (HR, 56.99; 95% CI, 21.53–150.88), MDR E. coli (HR, 36.83; 95% CI, 17.59–77.14), MDR K. pneumoniae (HR, 15.83; 95% CI, 5.17–48.48), and MDR A. baumannii (HR, 15.25; 95% CI, 7.58–30.69). With respect to isolation site, previous MDRGNB culture from urinary tract had the highest risk for subsequent MDRGNB urinary tract infection after discharge (HR, 18.45; 95% CI, 8.25–41.27) (Table 4).

Table 4 Species-Specific Risk for Subsequent Infection with Causative Multidrug-resistant Gram-negative Bacteria (MDRGNB) or Infection Site Identical to Prior MDRGNB Culture during Index Hospitalization

A total of 148 in hospital mortality events were observed during the 12-month follow-up period after discharge from index hospitalization; 53 (35.8%) of them were associated with subsequent MDRGNB infections. MDRGNB-CP patients had significantly lower cumulative probability of survival than MDRGNB-CN patients, irrespective of all-cause or subsequent MDRGNB infection-associated mortality (both log-rank tests, P < 0.001) (Fig. 1b and c). By Cox regression analysis, MDRGNB-CP during index hospitalization was an independent predictor for all-cause mortality (HR, 2.42; 95% CI, 1.67–3.50) and subsequent MDRGNB infection-associated mortality (HR, 4.88; 95% CI, 2.79–8.52), after controlling other potential confounders, such as age, malignancy, high Charlson comorbidity index, and long-term indwelling urinary catheter (Table 5).

Table 5 Univariate and multivariate cox regression analysis for the risk for mortality after discharge from index hospitalization

We further divided our MDRGNB-CP patients into 2 subgroups: MDRGNB surveillance culture positive only patients and MDRGNB clinical culture positive patients (all patients with positive MDRGNB clinical culture, including those with both positive MDRGNB clinical and surveillance culture). After controlling for potential confounders, the risk for subsequent MDRGNB infection were both significantly higher in the MDRGNB surveillance culture positive only patients (HR, 4.63; 95% CI, 2.36–9.10) and MDRGNB clinical culture positive patients (HR, 5.56; 95% CI, 3.77–8.20), compared to that of MDRGNB-CN patients. The higher risk for all-cause mortality and subsequent MDRGNB infection-associated mortality after index hospitalization, compared to those of MDRGNB-CN patients, remained significant for the MDRGNB surveillance culture only patients (all-cause mortality, HR, 2.29; 95% CI, 1.19–4.41; subsequent MDRGNB infection-associated mortality, HR, 3.44; 95% CI, 1.20–9.90) and for the MDRGNB clinical culture positive patients (all-cause mortality, HR, 2.46; 95% CI, 1.62–3.74; subsequent MDRGNB infection-associated mortality, 5.33; 95% CI, 2.96–9.60).


This study assessed the effects of prior MDRGNB colonization on subsequent infection risk and long-term outcome after hospital discharge and yielded three major findings. First, even in an environment that is expected to be free of or with low antibiotic selection pressure, the effect of previous MDRGNB colonization on the risk for subsequent MDRGNB infection remains significant and prolonged. Second, species-specific and isolation site-specific risks for the occurrence of subsequent MDRGNB infection were observed. The risk for subsequent infection was especially high for P. mirabilis and P. aeruginosa or genitourinary tract as colonization microorganisms or anatomical site, respectively. Third, prior MDRGNB colonization history independently predicted long-term survival of patients after hospitalization and directly contributed for more than one-third of late mortality. These findings are novel and important for first-line physicians to prepare for appropriate empirical antibiotics and timely infection control intervention in the treatment of previously hospitalized patients with community-onset infection.

Colonization and infection of antimicrobial-resistant bacteria are common in hospitalized patients and introduce significant risks for short-term morbidity and mortality. The effects of prolonged colonization and subsequent infections after hospital discharge should be comprehensively evaluated; however, only methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus were previously reported [23,24,25,26]. A retrospective study by Huang et al. found that 17.4% (31/178) of patients with newly identified MRSA colonization or infection developed subsequent MRSA infections that were first manifested after discharge from the index hospitalization during an 18-month follow-up period [24]. In the current study, we demonstrated that 48% (60/125) of MDRGNB colonized or infected patients developed subsequent MDRGNB infections in a 12-month follow-up period, with more than half of these subsequent infections caused by the same MDR bacterial species and culture site. The risk for subsequent MDRGNB infection was especially high within 3 months after hospital discharge. Furthermore, our study found that patients harboring MDRGNB was an independent predictor for subsequent mortality after discharge from index hospitalization. Because previous studies never evaluate post-discharge events for patients with positive MDRGNB culture during hospitalization, the effect of MDRGNB on community disease burden and patient outcome were therefore underestimated. This is the first study that demonstrated the prolonged effect of prior acquisition of antimicrobial-resistant bacteria on subsequent infections and mortality after initial hospitalization, with not only gram-positive bacteria but also GNB. As the prevalence of MDRGNB is continuously increasing in community and hospital environments [1, 2, 27, 28], our study findings highlight the urgent need for effective approaches to prevent the spread of MDRGNB in hospitals and to mitigate the MDRGNB colonization burden after hospitalization.

E. coli and A. baumannii were the most prevalent MDR species identified from patients during index hospitalization and at subsequent infections in this study. However, species-specific hazard risks for developing subsequent infection were higher for P. mirabilis and P. aeruginosa than those for E. coli and A. baumannii. This finding is important and deserves further exploration. A previous study by O’Fallone et al., which serial rectal surveillance cultures were obtained every 3–4 weeks from 33 elderly residents of a long-term care facility, found that multidrug-resistant P. mirabilis had significantly lower clearance rate (6.7%) and longer colonization duration than other non-Proteus MDR species [16]. However, they did not evaluate the effects from persistent colonization of P. mirabilis on the risk for subsequent infection. Our study results, by demonstrating the differential risk for subsequent infection after acquisition of different MDRGNB species, suggest the positive correlation between colonization persistence and risk for subsequent infection after hospital discharge. Further studies that explore the contributing factors for persistent MDRGNB colonization should be conducted to design effective decolonization strategies from infection control and prevention perspectives [29,30,31].

Though patients identified as harboring MDRGNB had significantly higher risk for developing subsequent MDRGNB infections, more than half of all subsequent MDRGNB infections occurred in patients who were not positive for MDRGNB in either initial surveillance cultures on admission or clinical cultures during hospitalization. These patients might have new MDRGNB colonization but were not detected by decision-driven clinical cultures during index hospitalization. This indicates the limitation of using clinical cultures alone in identifying patients with MDRGNB colonization. Other clinical characteristics, including old age, comorbid illnesses, and prior antibiotics exposure, also help clinicians in early suspicion of patients at risk for subsequent MDRGNB infections [10, 12, 13]. Clinicians should incorporate these data to guide their decision on using appropriate empirical antimicrobial therapy for patients with prior healthcare-associated exposure risk.

This study has several limitations. First, this was a single-center study. Thus, generalization from our findings requires further confirmation. Second, outcome data after index hospitalization were retrospectively collected and are therefore subject to information bias. Third, because not all study patients had completed a 12-month follow-up at our hospital, subsequent MDRGNB infection and mortality event that occurred elsewhere would not have been detected. Fourth, because the gastrointestinal tract is an important anatomical site harboring antimicrobial-resistant Enterobacteriaceae and P. aeruginosa [16, 32, 33], the lack of perianal swab cultures in active microbiological surveillance may have caused the underestimation of the MDR-GNB colonization rate and may have introduced an information bias and misclassification of study groups. Finally, we did not perform genotypic analysis for the 41 subsequent MDRGNB infection episodes that were caused by the same MDR bacterial species identified during index hospitalization. Therefore, the genotypic concordance between index hospitalization and subsequent infection isolates could not be confirmed.


The prolonged effects of prior MDRGNB colonization or infection significantly increase the risk for subsequent MDRGNB infection and mortality after hospitalization. P. mirabilis and P. aeruginosa as causative microorganisms or urinary tract as colonization site is a serious concern. Accurate assessment of the risk for MDRGNB-associated sequelae requires prolonged follow-up after discharge. Furthermore, studies aiming at the prevention of MDRGNB acquisition and eradication of colonization to reduce the prolonged effect of MDRGNB colonization are imperative.



Hazard ratio


Intensive care unit


Length of hospital stay


Multidrug-resistant gram-negative bacteria


Multidrug-resistant gram-negative bacteria culture negative


Multidrug-resistant gram-negative bacteria culture positive


Non-fermentative gram-negative bacilli


Outpatient Department


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This study was supported partly by grants from the Department of Health, Taiwan (DOH98-DC-1005).

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The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.

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W-P T, Y-C C, S-Y C conceived and designed the studies. W-P T, Y-C C, S-Y C and S-Y C contributed to acquisition and analysis of the data. The manuscript was prepared by W-P T and S-Y C. Y-C C and S-C C supervised the conduction of the trial and data collection. S-Y C takes responsibility for the paper as a whole. All authors read and approved the final manuscript.

Correspondence to Shey-Ying Chen.

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Fig. 2

Patient enrollment and classification flowchart. Abbreviations: OPD, outpatient department; MDRGNB, multidrug-resistant gram-negative bacteria; MDRGNB-CP, multidrug-resistant gram-negative bacteria culture positive; MDRGNB-CN, multidrug-resistant gram-negative bacteria culture negative

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Tseng, W., Chen, Y., Chen, S. et al. Risk for subsequent infection and mortality after hospitalization among patients with multidrug-resistant gram-negative bacteria colonization or infection. Antimicrob Resist Infect Control 7, 93 (2018).

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  • Multidrug resistance
  • Gram-negative bacteria
  • Colonization
  • Subsequent infection
  • Mortality